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POCCHIARI, F . and ROSSI, C . (1955), Proc. Roy. Soc. B. 143, 481 and subsequent papers. 6. BRESLOW, R. (1957),/. Amer. Chem. Soc. 79, 1762. 7. BRIN, M . , SCHOHET, S . S . and DAVIDSON, C . S . (1958),/. Biol. Chem. 230, 319. 8. COXON, R. V . and PETERS, R. A. (1950), Biochem. J. 46, 300. 9. COXON, R. V . , LIEBECQ, C . and PETERS, R. A. (1949), Biochem. J. 45, 320. 10. DREYFUS, P. M . and VICTOR, M . (1961), Amer. J. Clin. Nutr. 9, 414. 11. DRURY, A. , HARRIS, L. J. and MAUDSLEY, C . (1930), Biochem.

G . , CARLSON, W . E. and EVANS, C . A. (1941), Nutr. 21, 243. 20. GREEN, D . , HERBERT, D . and SUBRAHMANYAN, V . (1941), / . Biol. Chem. 21. 22. 23. 24. 25. 26. 27. 28. 29. 138, 327. GRIJNS, G . (1901), Geneesk. Tijdscht. Ned. Ind. 43, 3. GUBBER, C . J. (1961), Biol. Chem. 236, 3112. HAYASAKA, E. (1929), Tokohu,J. Exp. Med. 14, 85. HESS, W . E. (1921), Z. Physiol. Chem. 117, 284. HOLZER, H . and BEAUCHAMP, K. (1961), Biochem. Biophys. Acta 46, 225. HOLZER, H . (1961), Angew. Chem. 73, 721-727.

I had worked out also a formaldehyde azo test with a high specificity for the vitamin, which was useful in the isolation, and also a test based upon the use of the pigeon brain, which we called the catatorulin test. At that time also the concentrate which we had made in the course of the work proved of some value to others in the delineation of the other factors of the vitamin Β complex. As far as I know, we were the FIG. 14. Crystalline sulphate of thiamine with low melting point, 203°C. (Kinnersley, O'Brien & Peters, 1935).

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